Cancer Biology, Molecular Biology, Cell Migration, Cell Adhesion, Metastasis, Peptide Chemistry
The research emphasis in my laboratory is on deciphering the localized and transient signalling events which occur in cells during migration. Cell migration is orchestrated by a series of complex signalling cascades which requires crosstalk between cell surface receptors and components of the cell cytoskeleton. Understanding the molecular mechanisms that regulate cell migration is of important intellectual and clinical interest, and we believe this work may reveal fresh approaches to target cancer as well as developmental and neurodegenerative diseases. Research projects on-going in the laboratory range from the most fundamental, where the development of a better understanding of the molecular mechanism(s) regulating cell migration is the goal, to the very applied, where the identification of novel therapeutic targets and the design and synthesis of novel materials and surfaces to study cell behaviour is the desired endpoint. To help us achieve our goals, we are using a series of complimentary and synergistic cellular and molecular approaches and novel technologies including: 3D cell culture models, neuronal models, 2D ESI MS/MS, peptide array technology, label free live cell monitoring, qPCR and droplet qPCR, SEM, TEM and con-focal microscopy. In collaboration with Dr Tara Daltons group (Stokes Institute) we are applying novel gene expression techniques and droplet technology to decipher signaling pathways in breast and colon cancer.
More Information: CancerResearchUL
Dowling CM, Herranz Ors C, Kiely PA. Using real-time impedance based assays to monitor the effects of fibroblast derived media on the adhesion, proliferation, migration and invasion of colon cancer cells. Biosci Rep. 2014 Jun 17.
Patricia E. Collins, Patrick A. Kiely, and Ruaidhrí J. Carmody. Inhibition of Transcription by B Cell Leukemia 3 (Bcl-3) Protein Requires Interaction with Nuclear Factor κB (NF-κB) p50. J. Biol. Chem. 2014 289: 7059-7067. First Published on January 23, 2014
Dwane S, Durack E, O’Connor R, Kiely PA. RACK1 promotes neurite outgrowth by scaffolding AGAP2 to FAK. Cell Signal. 2013 Sep 19. doi:pii: S0898-6568(13)00273-8.10.1016/j.cellsig.2013.08.036.
Dwane S, Durack E, Kiely PA. Optimising parameters for the differentiation of SH-SY5Y cells to study cell adhesion and cell migration. BMC Res Notes. 2013 Sep 11;6(1):366.
Ron D, Adams DR, Baillie GS, Long A, O’Connor R, Kiely PA. RACK1 to the future–a historical perspective. Cell Commun Signal. 2013 Aug 1;11:53. doi: 10.1186/1478-811X-11-53.
Abreu CM, Kumar R, Hamilton D, Dawdy AW, Creavin K, Eivers S, Finn K, Balsbaugh JL, O’Connor R, Kiely PA, Shabanowitz J, Hunt DF, Grenon M, Lowndes NF. Site-specific phosphorylation of the DNA damage response mediator rad9 by cyclin-dependent kinases regulates activation of checkpoint kinase 1. PLoS Genet. 2013 Apr;9(4)
Shanley DK, Kiely PA, Golla K, Allen S, Martin K, O’Riordan RT, Ball M, Aplin JD, Singer BB, Caplice N, Moran N, Moore T. Pregnancy-Specific Glycoproteins Bind Integrin αIIbβ3 and Inhibit the Platelet-Fibrinogen Interaction. PLoS One. 2013;8(2):e57491.
Colleran A, Collins PE, O’Carroll C, Ahmed A, Mao X, McManus B, Kiely PA, Burstein E, Carmody RJ: Deubiquitination of NF-kappaB by Ubiquitin-Specific Protease-7 promotes transcription.
Proc Natl Acad Sci U S A 2013, 110:618-623.
Ryan RP, McCarthy Y, Kiely PA, O’Connor R, Farah CS, Armitage JP, Dow JM: Dynamic complex formation between HD-GYP, GGDEF and PilZ domain proteins regulates motility in Xanthomonas campestris. Mol Microbiol 2012, 86:557-567.
Neasta J, Kiely PA, He DY, Adams DR, O’Connor R, Ron D: Direct interaction between scaffolding proteins RACK1 and 14-3-3zeta regulates brain-derived neurotrophic factor (BDNF) transcription. J Biol Chem 2012, 287:322-336.